Abstract

ABSTRACT The chiral separation of abscisic acid enantiomers was studied by capillary electrophoresis using β-cyclodextrin (β-CD), γ-CD, hydroxypropyl-β-CD (HP-β-CD) and dimethyl-β-CD (DM-β-CD) as chiral selectors. The effect of the selector concentration, pH of the run buffer, field strength, temperature and capillary length on the chiral separation were investigated. Capillary zone electrophoresis of (±)-abscisic acid enantiomers was established. The interaction between entiomers and CDs is different due to different kinds of CDs and their substituted group. The size of the inclusion cavity also plays a critical role in the interaction. γ-CD, HP-β-CD and DM-β-CD form strong complexes with (±)-abscisic acid than β-CD. In the presence of γ-CD, the best R s was obtained.

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