Chiral ligand exchange capillary electrochromatography with dual ligands for enantioseparation of D,L-amino acids

Abstract

Utilizing block copolymers as coatings, a protocol of chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and developed with dual ligands for D,L-amino acids enantioseparation. Four block copolymers including poly maleic anhydride-co-styrene-co-N-methacryloyl-L-histidine methyl ester [P(MAn-St-MAH)], poly maleic anhydride-co-styrene-co-N-methacryloyl-L-lysine methyl ester [P(MAn-St-MAL)], poly maleic anhydride-co-styrene-co-N-methacryloyl-L-phenylalanine methyl ester [P(MAn-St-MAP)] and poly maleic anhydride-co-styrene-co-N-methacryloyl-L-threonine methyl ester [P(MAn-St-MAT)] were synthesized by reversible addition fragmentation chain transfer polymerization reaction. Key factors affecting the enantioresolution were optimized, including the concentration of Zn (II) central ion, pH value of buffer solution and monomers of the block copolymers. The enantioresolution of the proposed CLE-CEC system could be enhanced dramatically by employing P(MAn-St-MAH) as the immobilized chiral ligand and by coordinating the synergistic effect of free ligand in buffer solution. The principle of improved enantioresolution of the CLE-CEC system with dual ligands was discussed. Well enantioseparation was successfully realized with 7 pairs of D,L-amino acids enantiomers baseline separation and 5 pairs part separation. For quantitative analysis of D,L-alanine, a good linearity was established in the range of 9.4 μM to 1.5 mM (r2 = 0.997) with the limits of detection (LODs) 3.7 μM of D-alanine, 2.0 μM for L-alanine, and limits of quantification (LOQs) 9.0 μM for D-alanine and 6.0 μM for L-alanine. The peak area and migration time reproducibility (n = 6) were 4.1% and 3.5% for D-alanine, 3.7% and 3.1% for L-alanine. Further, the enzyme kinetics study of alanine aminotransferase was investigated with the constructed CLE-CEC system.