Abstract
A chiral liquid chromatographic method has been developed for separation of the enantiomers of abacavir sulfate, a nucleoside reverse transcriptase inhibitor for treatment of HIV. The enantiomers were resolved in a Chiralcel OD column with n-hexane–ethanol–trifluoroacetic acid, 92:8:0.1 (v/v), as mobile phase. The resolution between the enantiomers was not less than 3.5. The trifluoroacetic acid had a very important effect on the separation of enantiomers. The method was extensively validated to prove its suitability and robustness. Solution stability and mobile-phase stability were studied for 48 h and found to be stable during this period. The method was found to be suitable for use in quality-control laboratories for analysis of the commercial drug substance.
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