Abstract
The enantioselectivity of the interaction of a polyclonal anti-DNP (dinitrophenyl) antiserum with a variety of DNP-amino acids has been determined by an indirect noncompetitive ELISA and the results obtained have been compared with those of an indirect competitive ELISA. The competitive ELISA offers an advantage to determine the I 50 values representing the relative affinities of the investigated DNP-amino acid enantiomers. In all cases, the I 50 values obtained were different for the individual DNP-amino acid enantiomers. While the l-enantiomers of ϵ-DNP-lysine, δ-DNP-ornithine, DNP-alanine and DNP-proline show a higher relative affinity for the antibody combining site than the d-enantiomers, an inverse chiral recognition was found for DNP-phenylalanine. On account of the high sensitivity of the ELISA system, the determination of small differences in the enantiomer recognition by the anti-DNP antibody is possible.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.