Abstract
A novel capillary zone electrophoretic method based on the use of propanol-modified beta-cyclodextrin buffers has been developed; it unambiguously detects and quantitates the optical isomers of racemethorphan and racemorphan without prior derivatization. Detection limits in urine down to 20 ppb have been obtained. The method uses a solid-phase extraction recovery of analytes followed by ultraviolet detection. Separation conditions include the use of 60mM beta-cyclodextrin and 50mM sodium dodecyl sulfate in a buffer containing 20% 1-propanol.
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