Abstract
AbstractThe chiral separation of enantiomers is crucial for pharmacovigilance within drug discovery. Although a large number of prescribed medications are marketed as pure enantiomers, this is not always the case and many are in fact racemic mixtures. Drug scandals, such as that of Thalidomide in 1961, provide a clear example of the social and economic repercussions that can be caused by negligence of these chiral compounds. Two high performance liquid chromatography (HPLC) methods are presented to determine, separate and quantitate a commonly prescribed chiral beta blocker, (-)-Alprenolol. The first method utilises a chiral column to physically separate the two enantiomers of Alprenolol in 25 minutes, before quantitating with two detectors. Fluorimetry gave the better limit of detection of 0.16-0.41ng and a correlation coefficient of 0.999. The second method used an achiral column coupled with polarimetry to quantitate (-)-Alprenolol without the need for physical separation in 10 minutes. The limit of detection achieved was 27-37μg and demonstrated a correlation coefficient of -0.999.
Highlights
Beta blockers impede the effects of epinephrine and norepinephrine within cardiac and smooth muscle resulting in a decrease in heart rate and blood pressure [1]
Attempts at the resolution of enantiomeric forms of Alprenolol and other beta blockers has been done previously using capillary electrophoresis (CE) [12,13], micellar electrokinetic chromatography (MEKC) [14,15], capillary isotachophoresis coupled with nuclear magnetic resonance [16], nano-LC [11] and high performance liquid chromatography (HPLC) with an acid glycoprotein (AGP) column [3,17,18,19]
While HPLC is an analytical technique that is rarely surpassed in achieving separation, one of the most difficult challenges it has faced is perfecting the resolution of enantiomers [21]
Summary
Beta blockers impede the effects of epinephrine and norepinephrine within cardiac and smooth muscle resulting in a decrease in heart rate and blood pressure [1]. Attempts at the resolution of enantiomeric forms of Alprenolol and other beta blockers has been done previously using capillary electrophoresis (CE) [12,13], micellar electrokinetic chromatography (MEKC) [14,15], capillary isotachophoresis coupled with nuclear magnetic resonance (cITP/NMR) [16], nano-LC [11] and HPLC with an acid glycoprotein (AGP) column [3,17,18,19] These previous citations, have demonstrated various limitations such as poor sensitivity, inability to identify low purity chiral samples and lengthy method preparations [3,16,17,18,19]. LC grade water (H2O) was prepared by passing de-mineralised water through a Milli-Q filtration system (Millipore, Bedford, MA)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
