Abstract
I present a summary of the first reports of the preparation and analysis of "chiral acetate", versions of acetic acid that are chiral by virtue of the presence of the three isotopes of hydrogen on the methyl group: a proton, a deuteron, and a tritium atom. Next I describe how "chiral acetate" allows the determination of the steric course of the addition of acetate to malate, and the steric course of the biochemical formation and cleavage of citrate. I conclude with a review of two additional syntheses of "chiral acetate", and the use of this material in the determination of the steric course of the biochemical methyl transfer from (S)-adenosylmethionine (AdoMet or SAM). This work is a sophisticated tour de force that involves tracer levels of materials, the ultimate stereochemical phenomena, isotope effects, and the exquisite specificity of enzymatic reactions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
