Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns

Abstract

The genetic diversity of melon market types (Cucumis melo L., 2n = 2x = 24) in China, an important secondary center of diversity, has not been examined. Therefore, reference accessions from India and Africa, Crete/Greece, Japan, Europe, U.S.A., Spain, and 68 Chinese cultigens (fresh-market non-netted thin-skinned; non-netted thick-skinned; netted thick-skinned; and non-netted thin-skinned, and vegetable) were evaluated by using 17 10-mer RAPD primers (32 mapped loci), days to flower, sex expression, lateral-branch number, and fruit number and weight per plant. While Chinese thin-skinned melons differed from vegetable melon types only in sex expression, the U.S. Western Shipping market type reference accession “Top Mark” and Chinese thick-skinned melons were similar for all of the morphological traits examined. The average similarity (Jaccard Coefficient) between any two pairs of accessions examined as estimated by RAPD variation was 0.47 ± 0.14. Within-group genetic similarities ranged between 0.94 (thin-skinned type) and 0.08 (non-netted thick-skinned type). The average/standard deviation, maximum, and minimum similarity between any two Chinese reference accessions was 0.41 ± 0.13, 0.75, and 0.12, respectively. Cluster analysis partitioned accessions into two main branches consisting of Group Cantalupensis and Inodorus reference accessions (clade 1) and Chinese accessions (clade 2). A second cluster analysis partitioned China, India, and Africa accessions into one major group, and accessions from Japan, Europe, and U.S.A. into another. Results indicate that Chinese accessions are a rich source of genetic diversity for plant improvement, and that molecular assessments support previously described theoretical melon domestication patterns constructed from historical and archeological evidence.