Abstract
Liver injury, one of the causes of liver failure, is mainly due to T-cell-mediated immunity. Traditional Chinese medicine Jiedu Huayu granules are often used to suppress liver damage and improve liver function. The specific regulatory mechanism of Jiedu Huayu granules has not been fully studied, and its function in the immune system remains unclear. Therefore, in this study, the mechanism of Jiedu Huayu granules in the prevention of hepatic injury was studied in a rat model of hepatic injury induced by D-galactoside and lipopolysaccharide. The cytotoxic T lymphocytes (CTLs) in the peripheral blood were examined. Perforin, granule B, and PD1 expression in CTL increased after the induction of hepatic injury and could be reduced by Jiedu Huayu granules. Hepatic apoptotic factors OX62, FAS, and TNFR1 associated with CTL function were also reduced by Jiedu Huayu granules. These results suggested that Jiedu Huayu granules could inhibit the inflammatory response to relieve liver damage by mediating the T-cell immunity. Therefore, the discovery of the mechanism of action of Jiedu Huayu granules in the immune system could allow their use more effectively in clinical practice.
Highlights
E D-galactoside and lipopolysaccharide-induced liver injury model induced in rats by toxic doses mimic viral hepatitis and is widely used in drug screening studies against viral hepatitis and fulminant hepatic failure [4,5,6]
Us, our work focused on the evaluation of the effect of Jiedu Huayu granules on liver function, and our results suggested that it inhibited the inflammatory response to relieve liver damage by mediating the T-cell immunity
In patients with acute liver failure (ALF), cytotoxic T lymphocytes (CTLs) mediated by immune function can lead to an overimmune response, leading to liver failure. erefore, in order to detect the changes in CTL cells in ALF and Jiedu Huayu granule treatment, six rats were randomly selected from each group to detect the proportion of CTL cells in peripheral blood cells by flow cytometry. e results showed that, in the control group, the percentage of CD3+CD8+ CTL cells in rat peripheral blood was 3.16%, while in the ALF model group, the percentage of CD3+CD8+ CTL cells increased to 9.40%
Summary
E D-galactoside and lipopolysaccharide-induced liver injury model induced in rats by toxic doses mimic viral hepatitis and is widely used in drug screening studies against viral hepatitis and fulminant hepatic failure [4,5,6]. In this model, LPS stimulates Kupffer cells (specialized macrophages located in the liver) to secrete proinflammatory factors such as tumor necrosis factor TNF-α and various interleukins, and a series of inflammatory responses are the main causes of liver damage [7,8,9]. Us, our work focused on the evaluation of the effect of Jiedu Huayu granules on liver function, and our results suggested that it inhibited the inflammatory response to relieve liver damage by mediating the T-cell immunity
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