Chinese medicine Jiangzhuo mixture regulates glucose and lipid metabolism in obese rats throughTLR4/IκBα/NF-κB signaling pathway.

Abstract

To explore the mechanism of Chinese medicine Jiangzhuo mixture regulating glucose and lipid metabolism in obese rats. Thirty healthy male SD rats were randomly divided into normal control group, the model control group, and the Jiangzhuo mixture treatment group, with 10 rats in each group. The rats in the normal control group were fed with normal diet, the obesity model was induced by feeding high-adipose diet in the model control group and Jiangzhuo mixture treatment group, the rats in the treatment group were given with Jiangzhuo mixture 50 g·kg－1 by gavage. After 8 weeks of intervention, the blood glucose (GLU), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) levels were detected in three groups. Quantitative reverse transcription PCR were used to detect the expression levels of PR domain containing 16 (PRDM16) and uncoupling protein 1 (UCP1) in white and brown adipose tissue of rats in each group; Western blot was used to detect the expression of PRDM16 and UCP1 in the white and brown adipose of rats, and Toll-like receptor 4 (TLR4), IκBα and nuclear factor kappa-B (NF-κB) in the white adipose; immunohistochemical method was used to detect the expression of UCP1 protein in white and brown adipose tissue. Compared with the normal control group, the white adipose weight (P<0.01), white adipose coefficient (P<0.05) and Lee's coefficient (P<0.01) were significantly increased in the model control group; the contents of GLU, TC, TG and LDL-C were all increased, and the content of TG was significantly increased (P<0.05) in the model control group. The mRNA and protein expression levels of PRDM16 and UCP1 in white adipose and brown adipose were significantly decreased (P<0.05) in the model control group. Compared with the model control group, the white adipose weight and white adipose coefficient and Lee's coefficient were significantly reduced in the Jiangzhuo mixture treatment group (P<0.01), the levels of GLU, TC, TG, and LDL-C in the the treatment group were all reduced, and the content of TG was reduced more obviously (P<0.01); expression levels of PRDM16 and UCP1mRNA and protein were increased in brown and white adipose. Compared with the normal control group, the expression levels of TLR4, IκBα and NF-κB proteins in white adipose tissue of the model control group were significantly increased (all P<0.01), while the expression levels of these proteins in the Jiangzhuo mixture treatment group were significantly lower than those in the model control group (all P<0.05). Jiangzhuo mixture can alleviate high-adipose diet-induced increase in body adipose, abnormal expression of biochemical indexes and promote the expression of key transcription factor UCP1 and PRDM16 in white and brown adipose by regulating TLR4/IκBα/NF-κB signaling pathway in obese SD rats.