Abstract
Objective Lung cancer is one of the major causes of cancer deaths worldwide, and the five-year survival still remains low despite the improvement of screening, prevention, and treatment methods. Chinese herbal medicines have been widely used for tumor prevention and treatment. Miao-Yi-Ai-Tang (Miao) is a novel herbal formulation and shows a potential anticancer effect. Materials and Methods. Human Small Cell Lung Cancer Cell was used for study in vitro. After treatments by Miao and Cisplatin (DDP), the invasion, migration, proliferation, and apoptosis of cells were detected by transwell, wound healing, CCK-8, and flow cytometry, respectively. The expression of β-catenin, AXIN, and c-myc was detected by qRT-PCR and immunohistochemistry staining. Western blotting was applied for measuring the protein expression of β-catenin, AXIN, and c-myc was detected by qRT-PCR and immunohistochemistry staining. Western blotting was applied for measuring the protein expression of Results We found that Miao could inhibit invasion, migration, and proliferation and promote apoptosis of human lung cancer cells. Meanwhile, Miao and DDP presented synergy regulating the proliferation and apoptosis of lung cancer cells. The percentage of lung cancer cells in S and G2 stages was increased markedly by Miao. Besides, the expression of c-myc, AXIN, and β-catenin, AXIN, and c-myc was detected by qRT-PCR and immunohistochemistry staining. Western blotting was applied for measuring the protein expression of Conclusions Chinese herbal formulas Miao could suppress lung cancer through targeting the β-catenin/AXIN signaling pathway. Therefore, our findings may provide a novel strategy for the prevention and treatment of lung cancer.β-catenin, AXIN, and c-myc was detected by qRT-PCR and immunohistochemistry staining. Western blotting was applied for measuring the protein expression of
Highlights
Lung cancer is one of the major malignant tumors and characterized by high mortality throughout the world despite great development in diagnosis and treatments [1, 2]
We found that 20 times dilution of Miao presented a significant suppressive effect on cell proliferation of human lung cancer cells (NCI-H446) (Figure 1(a))
20 times dilution of Miao remarkably promoted the cell apoptosis of NCI-H446, and the promoting effect was dosedependent (Figures 1(b) and 1(c)). erefore, Miao might play an important role in regulating human lung cancer cells
Summary
Lung cancer is one of the major malignant tumors and characterized by high mortality throughout the world despite great development in diagnosis and treatments [1, 2]. Several reports indicated that the five-year survival of lung cancer is no more than 20% [3, 4]. Cisplatin (DDP) has been commonly used as first-line medicine to treat many tumors including lung cancer [5]. The major problem facing lung cancer patients is the resistance to DDP, and the mechanisms underlying the chemotherapy resistance remain unclear. Several therapeutic strategies including Xeroderma pigmentosum [6], CCAT1 [7], and microRNA10b [8] have been considered to be a regulator of DDP resistance. There should be a long way before they could be used in the clinic
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