Abstract
Cervicitis is a common sexually transmitted disease. In recent years, the abuse of antibiotic in the treatment of cervicitis results in the emergence of antibiotic-resistant bacteria; alternative strategies are needed to be developed. In this research, we investigated the effects of Feilin Vaginal Gel (FVG), a Chinese herbal formula, on the treatment of cervicitis. Two cervicitis models were optimized using BALB/c mouse; one in vitro model was established in HeLa cells. In Chlamydia trachomatis-induced cervicitis model, the high level of bacterial loads, the inflammation in tissue, and the cytokines in serum could be observed. With the administration of FVG, the bacterial loads in cervical mucus and cervix tissue could be significantly inhibited in dose-dependent manners. The pathological injury of cervix and vagina, as well as the levels of IL-2, IL-17, and MCP-1 in serum, could be mitigated by FVG. FVG reduced the number of inclusion induced by C. trachomatis in HeLa cells. In addition, the histological damage in Escherichia coli and Staphylococcus aureus-induced cervicitis model could be reduced by FVG. These results suggest that FVG is capable of treating cervicitis through the inhibition of pathogens and the regulation of host immune responses. FVG may contribute as an alternative agent for the treatment of cervicitis.
Highlights
Cervicitis is a common sexually transmitted disease with an inflammatory condition of the uterine cervix [1]
The swabs with cervical mucus were dispersed with an equal volume of PBS, the level of C. trachomatis N in PBS was analyzed with enzyme-linked immunosorbent assay (ELISA) kit (Meilian, Shanghai, China) according to the manufacturer’s instruction
The reticulate bodies (RBs) begin to multiply after 7-21 days
Summary
Cervicitis is a common sexually transmitted disease with an inflammatory condition of the uterine cervix [1]. After treatment with the antibiotic, 10%-20% of patients will suffer from reinfection within one year, which may be due to the absence of protective immunity against Chlamydia [10]. Considering these conditions, alternative strategies are needed to be developed. Feilin Vaginal Gel (FVG) is developed from a clinically used Chinese medicine formula that is used to treat the mucopurulent cervicitis. Mouse cervicitis models were established to evaluate the treatment of FVG. After being infected by the mixture of Escherichia coli and Staphylococcus aureus, the treatment of FVG was evaluated through the histological examination of the cervix
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