Abstract
Many kinds of Chinese herb had been confirmed to have the character of anti-tumor, clinical reports about anti-tumor effects of Chinese herb had also been found in recent years, but most of the reports were focused on the clinical treatment of effectiveness for Chinese herb, on the other hand, review about Chinese herbal related with molecules on cancer-cell-apoptosis was seldom, many scientists could not believe such kinds of clinical describes about anti-tumor effects for Chinese herb, because these describes were lack of molecular biology evidence. Kanglaite(KLT) injection is an anti-tumor new drug which extracts from Chinese medicine-coix seed with modern advanced pharmaceutical technology, it is also a new biphase extended-spectrum anticancer medicine, the food and drug administration(FDA) of United States also approved a phase II trial of KLT to test its efficacy in treating non-small-cell lung cancer. Some studies show it could inhibit some anti-apoptotic gene and activate some pro-apoptotic gene, its injection solution is one of the new anticancer medicine that can significantly inhibit a various kinds of tumor cells, so it has become the core of research that how to further explore KLT injection to promote tumor cell apoptosis by impacting on related genes. In this review, the relationship between KLT and some tumor cell apoptosis molecules had been discussed and reviewed generally.
Highlights
Journal of Experimental & Clinical Cancer Research 2008, 27:31 http://www.jeccr.com/content/27/1/31 of cells, so the proliferation of tumor cell was inhibited, at the same time it can activate some pro-apoptotic factor, and further lead to apoptosis [8]
Wei CY [12] observed 34 cases' hepatoma carcinoma cell that cultivated in vitro and their changes after treated with KLT, the results indicated that, compared with the control group, the apoptosis of hepatoma carcinoma cell in KLT experimental group is very significant, there was significant difference between them, at the same time, the labeling index of wild type p53 in the treat group is (8.39 ± 1.42)%, but the labeling index of wild type p53 in the control group (2.11 ± 0.97)%, there was significant difference between these two groups. the phenomenon recorded here is the same as the studies of Wang JJ, Guo JW and Bao Y which had indicated [8,15,16]
When studying the KLT- induced the apoptosis of cancer cell(HL60), Li Y [19] make use of RT-PCR to detect the the gene expression of bcl-2, there was no significant change in genetic transcription after 24 hours when using KLT at 10 ul/ml
Summary
The study of KLT-induced apoptosis is focused on the above-mentioned gene. In short, KLT induce apoptosis of cancer cell by way of up regulating the expression of p53 gene, Fas gene, Caspase-3, PCNA, p21WAFI/CIPI and down regulating the expression of cyclin A, cyclin E1, cyclin F gene. Based on these characteristics, KLT has been used in the treatment on many kinds of malignant tumor, and the clinical effect show that KLT can repress tumor directly, improve the quality of life obviously, enhance the patient's immunity, and enhance the effectiveness of chemotherapy and reduce side effects [45,46,47], which is consistent with its pharmacology above-mentioned the relationship between the apoptosis of hepatoma carcinoma cell and gene is further studied, these research can not fully reveal the mechanism of KLTinduced apoptosis, and its experimental result is still preliminary, the trend of using KLT to prevent and cure liver cancer is already formed, and with the development of modern medical technologies, its mechanism will undoubtedly be revealed in the near future. Just as the result of experimental treatment on hepatoma of mice that we have done [44], we found that KLT can made the cancer cells stop in the G2+M phase of cell life circle, and prevent them form entering the G0 and G1 phase. so it can induce the apoptosis and suppress growth of cells without any effect on the surrounding normal tissues or causing Inflammatory reaction which are unique characteristics. based on these characteristics, KLT has been used in the treatment on many kinds of malignant tumor, and the clinical effect show that KLT can repress tumor directly, improve the quality of life obviously, enhance the patient's immunity, and enhance the effectiveness of chemotherapy and reduce side effects [45,46,47], which is consistent with its pharmacology above-mentioned the relationship between the apoptosis of hepatoma carcinoma cell and gene is further studied, these research can not fully reveal the mechanism of KLTinduced apoptosis, and its experimental result is still preliminary, the trend of using KLT to prevent and cure liver cancer is already formed, and with the development of modern medical technologies, its mechanism will undoubtedly be revealed in the near future. and KLT will bring new hope for the treatment of cancer and the protection of normal tissues with the more sufficient evidence of its effect on liver cancer and other tumor in clinical trials
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