Chimerism patterns of long-term stable mixed chimeras post-HSCT in patients with non-malignant diseases

Abstract

Event Abstract Back to Event Chimerism patterns of long-term stable mixed chimeras post-HSCT in patients with non-malignant diseases Arwen Stikvoort1, 2*, Jens Gertow1, 2, Mikael Sundin3, 4, Mats Remberger1, 2, Jonas Mattsson1, 2 and Michael Uhlin1, 2 1 Karolinska Institutet, Laboratory Medicine, Sweden 2 Karolinska University Hospital, Centre for Allogeneic Stem Cell Transplantation (CAST), Sweden 3 Karolinska Institutet, Clinical Sciences, Intervention and Technology (CLINTEC), Sweden 4 Karolinska University Hospital, Astrid Lindgren Children’s Hospital, Sweden Introduction. Long-term stable mixed chimerism (MC) is a rare phenomenon after hematopoietic stem cell transplantation (HSCT) characterized by 5-95% residual recipient hematopoietic cells. The mechanisms directing hematopoietic recovery to MC in some patients and to full donor chimerism (DC) in others are poorly understood. In this study we compared DC patients with long-lasting stable MC patients for a median of 9.5 years (range 5 – 16.5) post-HSCT in patients with non-malignant diseases. Methods. Chimerism analysis was performed for all patients at regular intervals post-HSCT on peripheral blood samples. The cell lineages analysed were T-lymphocytes (CD3), B-lymphocytes (CD19) and myeloid cells (CD33). Results. Several factors significantly associated with the likelihood of stable MC development were identified by univariate analysis, e.g. younger donor age, sibling donor and conditioning regimen. Despite a limited patient cohort, our multivariate analysis could confirm that a sibling donor was associated with stable MC development. Furthermore, development of acute-graft-vs.-host disease (aGvHD) and blood stream infection (BSI) was significantly more prevalent in the DC patient group. Additionally, significant fluctuations in the recipient:donor chimerism ratio decreased over time after HSCT in MC patients. Discussion. Sibling donor cells are a closer HLA and minor histocompatibility antigen match than unrelated donor cells and as thus will be less prone to induce immune-mediated killing of recipient cells. Additionally, milder conditioning regimens may also stimulate MC development. Furthermore, MC was not linked to a worse prognosis, as both aGvHD and BSI development were more prevalent in the DC group. Keywords: Hematopoietic Stem Cell Transplantation, Graft vs Host Disease, Sepsis, mixed chimerism, chimerism analysis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Stikvoort A, Gertow J, Sundin M, Remberger M, Mattsson J and Uhlin M (2013). Chimerism patterns of long-term stable mixed chimeras post-HSCT in patients with non-malignant diseases. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00513 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 May 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Arwen Stikvoort, Karolinska Institutet, Laboratory Medicine, Stockholm, Sweden, arwen.stikvoort@ki.se Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Arwen Stikvoort Jens Gertow Mikael Sundin Mats Remberger Jonas Mattsson Michael Uhlin Google Arwen Stikvoort Jens Gertow Mikael Sundin Mats Remberger Jonas Mattsson Michael Uhlin Google Scholar Arwen Stikvoort Jens Gertow Mikael Sundin Mats Remberger Jonas Mattsson Michael Uhlin PubMed Arwen Stikvoort Jens Gertow Mikael Sundin Mats Remberger Jonas Mattsson Michael Uhlin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.