Chimeric Viruses Expressing Primary Envelope Glycoproteins of Human Immunodeficiency Virus Type I Show Increased Sensitivity to Neutralization by Human Sera

Abstract

We constructed a number of HXB2 viruses chimeric for the gp120 glycoprotein derived from a number of viable molecular clones obtained from a primary isolate. Comparative biological characterization of the parental primary viruses with the gp120.HXB2 chimeras demonstrated identical patterns of cell tropism and cytopathicity. Furthermore, both parental and chimeric viruses were insensitive to neutralization by sCD4 and a panel of conformation-dependent monoclonal antibodies, demonstrating that transfer of the gp120 protein alone was sufficient to confer a “neutralization-resistant” phenotype to the T-cell-adapted clone HXB2. We assessed the contribution of gp120 epitopes to the neutralizing immune response by comparing the sensitivity of these viruses to neutralization by a panel of sera from HIV-infected individuals. Seven of eleven sera tested were able to neutralize HXB2 and two or more of the chimeric viruses; in contrast, only one serum neutralized more than one of the parental primary virus clones. The association of gp120–gp41 envelope at the surface of infected PBMC cultures was measured in the presence or absence of soluble CD4. No differences in CD4-induced gp120 dissociation were seen between the chimeric and parental virus-infected cultures. Since gp120 conformation appeared the same between primary and chimeric viruses, we suggest that the ability of human sera to neutralize the chimeric viruses may be mediated by epitopes within gp41.