Abstract
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Highlights
Breeding flocks must be free of Salmonella and early detection is extremely important to prevent the disease and its dissemination (Barrow, 1994)
After the first enzyme linked immunosorbent assay (ELISA) to detect Salmonella enterica subspecies arizonae infection has been described in turkeys (Nagaraja et al, 1984, Nagaraja et al, 1986), other reports have indicated the usefulness of different indirect ELISAs to detect circulating antibodies (Barrow, 1991)
Since the development of ELISA in the 1980s, they have been increasingly used in poultry flocks in the serological detection of invasive Salmonella serotypes associated with human food poisoning, such as S
Summary
Breeding flocks must be free of Salmonella and early detection is extremely important to prevent the disease and its dissemination (Barrow, 1994). Serological methods may be used to detect infection caused by a number of serotypes (Barrow, 1992). Since the development of ELISA in the 1980s, they have been increasingly used in poultry flocks in the serological detection of invasive Salmonella serotypes associated with human food poisoning, such as S.
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