Abstract
Ornithine transcarbamylase (OTC), one of the enzymes of the urea cycle, is detectable in some strains of chickens, although they have no functional urea cycle. The enzyme consists of three identical subunits of 36 kd and is present in mitochondria of the kidney. Using immunoabsorbent column chromatography, we found further evidence that the enzyme is detectable as a precursor form (40 kd) in chicken brain, heart, liver, pancreas, gizzard, small intestine, and breast muscle. When an extract of small intestine containing only precursor OTC was treated with a kidney extract, the precursor was converted into OTC. This suggests that there is a tissue-specific processing protease in the kidney which splits a peptide off the precursor, causing the expression of OTC activity in this organ. However, the reason why the enzyme or its precursor is expressed in these organs is not known. The results of this study suggest that, unlike mammals, chickens are more organ specific with regard to the ability to incorporate precursor OTC into mitochondria.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
