Chicken model of steroid-induced bone marrow adipogenesis using proteome analysis: a preliminary study

Sing Chung Li,Yun Ho Lin,Ching Yu Lin,Chia Chun Chen,Tzong Fu Kuo,Way Neng Lin,Wing P Chan

doi:10.1186/1477-5956-8-47

Abstract

BackgroundSteroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow. This may be a contributing factor in some forms of osteonecrosis. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins were identified by MALDI-TOF MS.ResultsOne MP-induced chicken died of overdose anesthesia. Methylprednisolone-induced proliferation of adipose tissue and new bone formation were found on histologic examination. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins.ConclusionsProteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens.

Highlights

Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow
In 1997, Cui and colleagues [2] first reported that significant adipogenesis and trabecular bone loss of the femoral head could be induced by injection of high-dose corticosteroids in a chicken model
We aimed to use a proteomic approach to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model, which has not been reported before

Summary

Introduction

Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow This may be a contributing factor in some forms of osteonecrosis. The proteomic technology of two-dimensional gel electrophoresis (2-DE) has been widely used in chickens [11], pigs [12], rats [13], rabbits [14], and humans [15,16]. This is currently the only technique that can be applied routinely to quantitative parallel expression profiling of large sets of complex protein mixtures [17]

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