Abstract
Background: Commercially available conventional growth medium for the culture of microbes are expensive, hence the need for alternative cheaper sources. Poultry waste, in the form of feather and blood, are of value in biotechnology because of their high protein content. Hence the primary aim of this study was to produce a cheaper peptone alternative from chicken feather protein hydrolysate (CFPH) and blood meal (BM). Methods: We monitored the growth of selected bacteria and fungi in different concentrations of medium produced from varying combination of peptone, CFPH and BM in order to determine the combination that produced maximum growth. Five different media, namely 100% peptone (control), 100% BM, 40% peptone + 60% CFPH, 40% BM + 60% CFPH and 20% peptone + 20% BM + 60% CFPH were prepared and used for the study. The different media were inoculated with 1 ml of each test organism ( Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida carpophila, Candida tropicalis and Pichia kundriavzevii) and their growth monitored for 10 h. Results:Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus grew best in the 100% peptone, Klebsiella pneumoniae grew best in 100 BM. The fungi species were observed to grow best in 100% peptone. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA, HCl, and HNO 3 gave the best growth of E. coli. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA) also gave the best growth of C. tropicalis and Klebsiella pneumoniae respectively. Conclusions: Overall, the 60% CFPH + 40% peptone combination showed the most potential as an alternative to peptone, especially for E. coli.
Highlights
Microbial culture medium is composed of different nutrients required by organisms for growth
In the TCA feather hydrolysate medium, after 10 hours, the organism recorded the highest growth in the 100% peptone medium, closely followed by 60% feather hydrolysate + 20% peptone + 20% blood, 60% feather hydrolysate + 40% peptone, and 100% blood in that order, while the least growth was observed with 60% feather hydrolysate + 40% blood (Figure 1)
It is opined that the use of alkaline hydrolysis inactivates of pathogens and prions such as transmissible spongiform encephalopathy (TSE) and bovine spongiform encephalopathy (BSE)
Summary
Microbial culture medium is composed of different nutrients required by organisms for growth. Available conventional growth medium for the culture of microbes are expensive, the need for alternative cheaper sources. The primary aim of this study was to produce a cheaper peptone alternative from chicken feather protein hydrolysate (CFPH) and blood meal (BM). The different media were inoculated with 1 ml of each test organism ( Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida carpophila, Candida tropicalis and Pichia kundriavzevii) and their growth monitored for 10 h. The 60% CFPH + 40% peptone combination The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA) gave the best growth of C. tropicalis and Klebsiella pneumoniae respectively. Conclusions: Overall, the 60% CFPH + 40% peptone combination showed the most potential as an alternative to peptone, especially for E. coli
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
