Chick embryo lethal orphan (CELO) virus-induced early and late polypeptides

Abstract

Polypeptides of chick embryo lethal orphan virus (an avian adenovirus) were analyzed with SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Virions ( p = 1.338 g/cm 3) and virion-like particles having lower density (light particles, p = 1.289 g/cm 3) were composed of at least 11 and 14 types of polypeptides, respectively. At least 23 virus-induced polypeptides in infected cells (primary monolayer culture of chicken kidney cells) were detected; eight polypeptides (early polypeptides) were synthesized even in the presence of 1-β- d-arabinofuranosyl cytosine, but the others (late polypeptides) were not. On the basis of migration in SDS-PAGE, 5 polypeptides were found to be common to virions and infected cells; 5, to light particles and infected cells; 10, to virions and light particles; and 4, to all three. Scarcely any of the early polypeptides were found in the soluble fraction when extracted with the low salt buffer. Some of the early polypeptides were solubilized with the high salt buffer. Other early polypeptides were solubilized by sodium deoxycholate; three of these were found in the so-called “M-band,” as are early polypeptides of human adenoviruses. An experiment with radioactive glucosamine indicated that one of early polypeptides found in the M-band was a glycoprotein.