Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications.

Anna Guller,Vlada Rozova,Yi Qian,Liuen (Olivia) Liang,Inga Kuschnerus,Aleksandra Polikarpova,Yin Yao,Alfonso Garcia-Bennett,Ewa M Goldys,Zahra Khabir,Annemarie Nadort,Andrei V Zvyagin

doi:10.3390/biomedicines9111578

Abstract

Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-specific micrometastases of triple-negative breast cancer (TNBC) that is fully implemented in a cost-efficient chick embryo (CE) experimental platform. The model was built as three-dimensional (3D) tissue engineering constructs (TECs) combining human MDA-MB-231 cells and decellularized CE organ-specific scaffolds. TNBC cells colonized CE organ-specific scaffolds in 2–3 weeks, forming tissue-like structures. The feasibility of this methodology for basic cancer research, drug development, and nanomedicine was demonstrated on a model of hepatic micrometastasis of TNBC. We revealed that MDA-MB-231 differentially colonize parenchymal and stromal compartments of the liver-specific extracellular matrix (LS-ECM) and become more resistant to the treatment with molecular doxorubicin (Dox) and Dox-loaded mesoporous silica nanoparticles than in monolayer cultures. When grafted on CE chorioallantoic membrane, LS-ECM-based TECs induced angiogenic switch. These findings may have important implications for the diagnosis and treatment of TNBC. The methodology established here is scalable and adaptable for pharmacological testing and cancer biology research of various metastatic and primary tumors.

Highlights

Metastases remain the leading cause of cancer-related deaths and one of the biggest challenges in oncology [1,2]
The majority of decellularized organs, except the brain, preserved the shape reflecting to the original one, while the matrix loosened to a certain extent
These results show that compared to the measurements on ED8, the chorioallantoic membrane (CAM) that developed naturally underwent detectable angiogenesis, while the chick embryo (CE) liver acellular organ-specific scaffolds (AOSSs) did not enhance this at the level of statistical significance

Summary

Introduction

Metastases remain the leading cause of cancer-related deaths and one of the biggest challenges in oncology [1,2]. Circulating tumor cells can escape from the vasculature (extravasation) in a distant organ and attach there (arrest/homing) to form a secondary colony [3]. These colonies are avascular groups of cancer cells, 0.2–2 mm in size, termed micrometastases [4]. If not destroyed by the immune system, micrometastases may either stay dormant or progress to bigger, bloodperfused macrometastases (>2 mm) [4,5,6] This conversion from micrometastases to massive secondary neoplasms is driven by a tumor-induced acceleration of blood vessel growth in the vicinity of the cancer cell colony (“the angiogenic switch”) [7,8]

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Results

Discussion

Conclusion