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Abstract
The chick embryo area vasculosa is an extraembryonic membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review article analyzes the possibility to use the area vasculosa as an in vivo assay for the screening of putative angiogenic and anti-angiogenic molecules in alternative to the chorioallantoic membrane, more useful to study tumor growth, angiogenesis, and metastasis, and the angiogenic activity of acellular scaffolds and organoids.
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