Chemotherapy for rat glioma using adriamycin-N-succinyl chitosan sustained release nanometer particles

Abstract

Objective To observe the in vitro and in vivo releasing effects of adriamycin-N-succinyl chitosan(SCS)sustained release nanometer particles following implantation,and evaluate the local chemotherapeutic effect on rats bearing C6 gliomas. Methods The matrix with adriamycin was prepared.Gelatin and agar were used as major materials.The releasing effects of adriamycin-SCSsustained release nanometer particles were determined in artificial cerebrospinal fluid or in 20 normal rats brains by the method of fluorometry.The median survival time(MST)was measured in 20 SD rats bearing intracranial C6 gliomas, and the inhibitory effect of regional administration of adriamycin-SCS on growth of subcutaneous tumors was evaluated in 20 rats. Results Constant-release of adriamycin-SCS lasted over 30 days in artificial cerebro-spinal fluid or normal rabbit brains.Local controlled release of adriamycin could prolong the survival of rats bearing C6 gliomas with the MST being 35 days. Local controlled release of adriamycin could significant inhibit the growth of the subcutaneous tumors. As compared with control group, the growth of the subcutaneous tumors was slowed down or ceased in experimental group.There was statistically significant difference in tumor volume between the two groups(χ2= 7. 68,P<0. 05).There was significant difference in apoptosis index between control group(6. 4%) and experimental group(59. 3%, χ2= 23. 59,P<0. 05). Conclusion With high regional concentration and avoidance of toxicity of systemic chemotherapy, control released chemotherapy of adriamycin-SCS sustained nanometer particles remarkably inhibited the growth of C6 gliomas. Key words: Glioma; Adriamycin; N-succinyl chitosan; Sustained release particles