Abstract
Three cytotypes (2n = 18; 2n = 27; 2n = 36) ofCynodon dactylon were found to occur in Punjab plains. Evidences from biochemical analyses were sought to distinguish these otherwise morphologically indistinguishable cytotypes. It was observed that the presence or absence of β-alanine and threonine in the flag leaves could be effectively used as a marker. Tetraploids had highest acid invertase activity which was accompanied with higher concentrations of hexoses and always with reduced concentration of sucrose. Acid invertase activity was least in triploids. Tetraploids were richer in protein content than triploids and had almost double the quantity present in diploids.
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