Chemotaxis-Related Factors Are Dysregulated In Bone Marrow Mesenchymal Stem Cells (MSCs) In Multiple Myeloma Patients

Abstract

AbstractAbstract 5010 Abstract Objective:This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor(HGF), stromal-derived factor-1(SDF-1), Chemokine (C-C motif) ligand 2 (CCL2) and interleukin-8(IL-8) in bone marrow mesenchymal stem cells (MSC) from multiple myeloma (MM) patients. Methods:The real time quantitative polymerase chain reaction(RQ-PCR)technique was used to detect the mRAN expression levels of HGF, SDF-1, CCL2 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients compared with 9 controls. Results:The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients, as compared with control (P < 0.01). However, the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients, as compared with control (P < 0.05). There was no significant difference in the mRNA expression levels of CCL2 and IL-8 between MM and control cohorts (P > 0.05). Conclusion:The research suggests that multiple chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysrelugulated in MM patients, which might result from the interplay between MM cell and MSC. Disclosures:No relevant conflicts of interest to declare.