Abstract
Free-living large amoebae have been shown to exhibit directed chemotactic movement towards hydra tissue and a water-soluble fraction extracted from commercial crude papain. The chemotactic responses are produced by the local formation of pseudopods and their subsequent extension. The chemotactically active substances have been shown to be positively charged macromolecules, and to act on the amoeba surface by combining with the mucous coat of amoebae. The mode of action of the chemotactic agents is discussed, and a speculative hypothesis is proposed for the mechanism of chemotaxis in amoebae in terms of local area growth of the cell membrane.
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