Chemotactic response of marine bacteria to the extracellular products of Synechococcus and Prochlorococcus

Abstract

The cyanobacterial genera Prochlorococcus and Synechococcus are key phototrophic organisms in the open ocean, and ecological interactions between these groups and heterotrophic bacteria have fundamental importance for marine carbon and nutrient cycling. We applied a microfluidic chemotaxis assay to study the chemotactic response of 3 marine bacterial isolates (Pseudoalteromonas haloplanktis, Vibrio alginolyticus and Silicibacter sp. TM1040) to the chemical products of Synechococcus elongatus and Prochlorococcus marinus MED 4Ax. In the ocean, chemi- cal products from these cyanobacteria may be released into the water column via extracellular exu- dation or cell lysis. Strong and rapid chemotactic responses by all 3 bacterial strains occurred in reac- tion to the Synechococcus products. P. haloplanktis cells exhibited the strongest attraction, accumulating within the chemoattractant band in concentrations of up to 9-fold above background levels. Positive chemotaxis to Prochlorococcus chemical products also occurred, with P. haloplanktis and Silicibacter sp. TM1040 exhibiting the strongest responses. These observations indicate that marine bacteria can exhibit behavioural responses to the chemical products of Synechococcus and Prochlorococcus, which may support ecological associations between these important populations of marine prokaryotes in the environment.