Chemotactic Factor for Polymorphonuclear Leukocytes Derived from Human Lung Giant Cell Carcinoma Lu65 Cells

Abstract

A chemotactic factor for polymorphonuclear leukocytes was found in the culture fluid of human lung giant cell carcinoma cell line Lu65. This factor was defined as chemotactic one by checkerboard analysis. Partial purification of the chemotactic factor was performed by DEAE-Sepharose and gel filtration with Sephadex G-100, which showed it to be basic and low molecular weight. The factor was also found in the filtrate of culture fluid passed through a Centriflo CF25 filter, thus indicating a molecular weight of less than 25kDa. Furthermore, three cell clones having higher activity of chemoattractant were selected from the original cell line.