Chemotactic and candidacidal responses of rabbit alveolar macrophages during postnatal development and the modulating roles of surfactant in these responses.

Abstract

Chemotactic responses of alveolar macrophages from 1-, 7-, and 28-day-old rabbits to various concentrations of endotoxin-activated serum and n-formyl-methionyl-phenylalanine were tested utilizing both blind well and agarose plate assay systems. A dramatic increase in both the chemotactic response and responsiveness to various concentrations of chemoattractant was observed during postnatal maturation. The pattern of result was similar with both methods of assay. An age-related increase was also found to occur in the candidacidal activity of alveolar macrophages in contrast to their phagocytic uptake, which showed no age-related increases. Furthermore, the decreased function of macrophages from newborn animals correlated with a morphologically and biochemically less mature cell population which contained large amounts of phagocytosed surfactant-related material. Moreover, pretreatment of macrophages from 7- and 28-day-old animals with vesicles of surfactant-related material resulted in decreases in both chemotactic and candidacidal activity, with a paradoxical increase in their phagocytic activity. The resulting activities were similar to those of macrophages from 1-day-old animals treated with buffer alone. These data suggest that there is an age-related increase in the chemotactic and candidacidal activity of alveolar macrophages during maturation and that the decreased activity of macrophages from newborn animals is related in part to the large amount of surfactant-related material present at that time.