Abstract
Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.
Highlights
Insect chemosensory systems interpret and react to various environmental chemical factors, and impact various life cycle processes, including feeding, predator avoidance, and reproductive behavior [1,2]
The results show that CchiOBPs had several putative one-to-one orthologous relationships with these species, and only
The results show that CchiOBPs had several putative one-to-one orthologous relationships with these species, and only CchiOBP1 belongs to the Plus-C subgroup; the other CchiOBPs were clustered in the Classic subgroup
Summary
Insect chemosensory systems interpret and react to various environmental chemical factors, and impact various life cycle processes, including feeding, predator avoidance, and reproductive behavior [1,2] Recognition of these behaviors frequently involves a series of genes, including those that encode odorant binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs) [3,4,5]. These proteins participate in extremely complex biochemical reactions in various tissues to ensure accurate delivery of chemical signals [6]. It provided the basis for proteomics analysis [42] and searching the potential control strategies [43]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
