Abstract
Tea grey geometrid (Ectropis grisescens), a devastating chewing pest in tea plantations throughout China, produces Type-II pheromone components. Little is known about the genes encoding proteins involved in the perception of Type-II sex pheromone components. To investigate the olfaction genes involved in E. grisescens sex pheromones and plant volatiles perception, we sequenced female and male antennae transcriptomes of E. grisescens. After assembly and annotation, we identified 153 candidate chemoreception genes in E. grisescens, including 40 odorant-binding proteins (OBPs), 30 chemosensory proteins (CSPs), 59 odorant receptors (ORs), and 24 ionotropic receptors (IRs). The results of phylogenetic, qPCR, and mRNA abundance analyses suggested that three candidate pheromone-binding proteins (EgriOBP2, 3, and 25), two candidate general odorant-binding proteins (EgriOBP1 and 29), six pheromone receptors (EgriOR24, 25, 28, 31, 37, and 44), and EgriCSP8 may be involved in the detection of Type-II sex pheromone components. Functional investigation by heterologous expression in Xenopus oocytes revealed that EgriOR31 was robustly tuned to the E. grisescens sex pheromone component (Z,Z,Z)-3,6,9-octadecatriene and weakly to the other sex pheromone component (Z,Z)-3,9-6,7-epoxyoctadecadiene. Our results represent a systematic functional analysis of the molecular mechanism of olfaction perception in E. grisescens with an emphasis on gene encoding proteins involved in perception of Type-II sex pheromones, and provide information that will be relevant to other Lepidoptera species.
Highlights
In insects, chemical cues are regarded as language and play significant roles in regulating feeding, mating, and ovipositing (Zhou, 2010)
Little is known about the molecular mechanisms regulating E. grisescens olfaction because of the paucity of sequence data for olfaction genes from E. grisescens
The transcriptomes of female antennae (FA) and male antennae (MA) of E. grisescens were sequenced with two independent biological replicates
Summary
Chemical cues are regarded as language and play significant roles in regulating feeding, mating, and ovipositing (Zhou, 2010). Insect antennae, which are covered with several different types of chemosensory sensilla, are the principal chemosensory organs. Olfactory signal transduction starts with the recognition of odor molecules by olfactory receptors, such as odorant receptors (ORs) and ionotropic receptors (IRs) bound to olfactory receptor neuron (ORN). The ORNs that are located within chemosensory sensilla are surrounded by aqueous lymphatic fluid. Water-soluble carriers are required to transport lipophilic compounds through the sensilla lymph. Odorantbinding proteins (OBPs) and chemosensory proteins (CSPs) enhance the solubility of odors and deliver them to the olfactory receptors
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