Abstract
BackgroundColon cancer is the third most commonly diagnosed cancer and the second leading cause of cancer mortality worldwide. Chalcone and its derivatives are reported to exhibit anti-cancer effects in several cancer cell lines, including colon cancer cells. In addition, chalcones have advantages such as poor interaction with DNA and low risk of mutagenesity. In our previous study, a group of chalcone derivatives were synthesized and exhibited strong anti-inflammatory activities. In this study, we evaluated the anti-cancer effects of the chalcone derivative, L2H17, in colon cancer cells.MethodsThe cytotoxicities of L2H17 on various colon cancer cell lines were investigated by MTT and clonogenic assay. Cell cycle and apoptosis analysis were performed to evaluate the molecular mechanism of L2H17-mediated inhibition of tumor growth. Also, scratch wound and matrigel invasion experiments were performed to estimate the cell migration and invasion after L2H17 treatment. Finally, we observed the anti-colon cancer effects of L2H17 in vivo.ResultsOur data show that compound L2H17 exhibited selective cytotoxic effect on colon cancer cells, via inducing G0/G1 cell cycle arrest and apoptosis in CT26.WT cells. Furthermore, L2H17 treatment decreased cell migration and invasion of CT26.WT cells. In addition, L2H17 possessed marked anti-tumor activity in vivo. The molecular mechanism of L2H17-mediated inhibition of tumor promotion and progression were function through inactivated NF-κB and Akt signaling pathways.ConclusionsAll these findings show that L2H17 might be a potential growth inhibitory chalcones derivative for colon cancer cells.
Highlights
Colon cancer is the third most commonly diagnosed cancer and the second leading cause of cancer mortality worldwide
We show that L2H17 possesses the best anti-tumor effect on colon cancer cell lines and lowest cytotoxicity on noncancerous cell among these four compounds
L2H17 shows selective cytotoxic effect on colon cancer cells We screened the cytotoxic effect of L2H17, L40H37, L6H21, and L48H37 in both human and mouse colon cancer cells through MTT assay
Summary
Colon cancer is the third most commonly diagnosed cancer and the second leading cause of cancer mortality worldwide. Chalcone and its derivatives are reported to exhibit anti-cancer effects in several cancer cell lines, including colon cancer cells. Chalcones have advantages such as poor interaction with DNA and low risk of mutagenesity. A group of chalcone derivatives were synthesized and exhibited strong anti-inflammatory activities. We evaluated the anti-cancer effects of the chalcone derivative, L2H17, in colon cancer cells. The mortality rate is decreasing as the result of improved detection, the incidence of colon cancer continues to increase [2]. Chalcones have been reported to exhibit several biological activities, including anti-tumor, anti-inflammatory, immunomodulatory, anti-bacterial, anti-malarial, anti-leishmanial, trypanocidal and nitric oxide inhibitory activity [6, 7]. Chalcone derivatives are reported to exhibit anti-
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
