Chemoprevention of N-Methyl-N-Nitrosourea Induced Mammary Carcinogenesis with Raloxifene and Melatonin: Metabolic Changes in Female Rats

M Chamilová ,E Adámeková,Peter Kubatka ,I Ahlers ,Bianka Bojková ,Karolina Kalicka ,E Ahlersová

doi:10.2754/avb200271020235

Abstract

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Highlights

The aim of this work was to determine the selected parameters of carbohydrate and lipid metabolism in the mammary carcinogenesis induced with N-methyl-N-nitrosourea (NMU) in two doses, each by 50 mg/kg of body weight with a 7-day interval between them within the postnatal days 43 and 54 in female Sprague-Dawley rats
Administration of RAL to NTB animals decreased the concentrations of malondialdehyde (MDA) in the serum and thymus, in the bone marrow in TB animals
The experimental carcinogenesis of mammary gland induced by chemocarcinogens DMBA or NMU is mostly dependent on the hormonal state of the host (W elsch 1985)

Summary

Introduction

The aim of this work was to determine the selected parameters of carbohydrate and lipid metabolism in the mammary carcinogenesis induced with N-methyl-N-nitrosourea (NMU) in two doses, each by 50 mg/kg of body weight with a 7-day interval between them within the postnatal days 43 and 54 in female Sprague-Dawley rats. RAL decreased the contents of CH and PL in the liver of NTB animals and increased the concentration of TG in both groups of animals. Administration of RAL to NTB animals decreased the concentrations of malondialdehyde (MDA) in the serum and thymus, in the bone marrow in TB animals. RAL plus MEL decreased the concentration of serum TG and PL and decreased the contents of CH and PL in the liver of TB as well as NTB animals. In the thymus and liver, combination of RAL+MEL decreased the MDA content compared with the RAL alone in NTB animals. The other efficient substance in the treatment of breast carcinoma is melatonin, the principal hormonal product of the pineal gland. The aim of our work was to find the effects of raloxifene, melatonin and their combination on the selected metabolic parameters in N-methyl-N-nitrosourea induced mammary carcinoma in female Sprague-Dawley rats and to compare their effects with the metabolic effects of “classical” antiestrogen tamoxifen alone or in combination with melatonin (Chamilová et al 2001)

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Conclusion