Abstract
Schiff-based complexes as a source of cancer chemotherapeutic compounds have been subjected to the variety of anticancer studies. The in-vitro analysis confirmed the CdCl2(C14H21N3O2) complex possess cytotoxicity and apoptosis induction properties in colon cancer cells, so lead to investigate the inhibitory efficiency of the compound on colonic aberrant crypt foci (ACF). Five groups of adult male rats were used in this study: Vehicle, cancer control, positive control groups and the groups treated with 25 and 50 mg/kg of complex for 10 weeks. The rats in vehicle group were injected subcutaneously with 15 mg/kg of sterile normal saline once a week for 2 weeks and orally administered with 5% Tween-20 (5 ml/kg) for 10 weeks, other groups were injected subcutaneously with 15 mg/kg azoxymethane once a week for 2 weeks. The rats in positive groups were injected intra-peritoneally with 35 mg/kg 5-Flourouracil four times in a month. Administration of the complex suppressed total colonic ACF formation up to 73.4% (P < 0.05). The results also showed that treatment with the complex significantly reduced the level of malondialdehyde while increasing superoxide dismutase and catalase activities. Furthermore, the down-regulation of PCNA and Bcl2 and the up-regulation of Bax was confirmed by immunohistochemical staining.
Highlights
Schiff-based complexes as a source of cancer chemotherapeutic compounds have been subjected to the variety of anticancer studies
There were no significant differences in rat body weight in any of the groups when compared with their respective vehicle groups (Table 3)
The results of this study demonstrated that the levels of superoxide dismutase (SOD) and CAT in the homogenised colon treated with the CdCl2(C14H21N3O2) complex significantly (p < 0.05) increased when compared with the cancer control group
Summary
Schiff-based complexes as a source of cancer chemotherapeutic compounds have been subjected to the variety of anticancer studies. The in-vitro analysis confirmed the CdCl2(C14H21N3O2) complex possess cytotoxicity and apoptosis induction properties in colon cancer cells, so lead to investigate the inhibitory efficiency of the compound on colonic aberrant crypt foci (ACF). Five groups of adult male rats were used in this study: Vehicle, cancer control, positive control groups and the groups treated with 25 and 50 mg/kg of complex for 10 weeks. All known colon carcinogens induce aberrant crypt foci (ACF) which is a preneoplastic lesions in rat colons, that induces molecular mutations in regulatory genes in the development of colon cancer[12]. In order to provide economical and simple tool for preliminary investigation of potential chemopreventive agents, the use of the ACF system for colon cancer studies has recently accelerated. Other premalignant lesions have been identified in the rat colon as possible biomarkers for further studies[14]
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