CHEMOMODULATORY EFFECT OFNIGELLA SATIVAAND GREEN TEA AGAINST FE-NTA-MEDIATED RENAL OXIDATIVE DAMAGE IN RAT

Abstract

Ferric nitrilotriacetate (Fe-NTA) is a potent nephrotoxic agent. The present study was designed to underline the protective mechanism of Nigella sativa (NS) and green tea (GT) against Fe-NTA-induced renal damage. Fe-NTA (5 mg Fe/kg body weight, i.p.) enhanced serum urea and creatinine levels, lactate dehydrogenase and N-acetyl-β-D-glucosaminidase activities. A depletion of renal glutathione content concomitant with a reduction in the activities of antioxidant and phase II metabolizing enzymes was detected. Lipid peroxidation, γ-glutamyl transpeptidase, adenosine deaminase and xanthine oxidase activities in renal tissue were markedly increased after Fe-NTA challenge. A significant deposition of iron in kidney and induction of serum prostaglandin E2 level were associated with the enhanced oxidative damage. Pretreatment of rats with either NS oil (2 mL/kg) or GT extract (100 mg/kg) by gavage for 7 consecutive days ameliorated most of the biochemical and histopathological changes induced by Fe-NTA. In conclusion, NS and GT can serve as potent chemopreventive agents to suppress oxidant-induced tissue injury and DNA damage. PRACTICAL APPLICATION Various therapeutic effects, such as anti-inflammatory, anticancer, antidiabetic and antimicrobial effects have been described for Nigella sativa and green tea. The present study is an endeavor in the direction of determining their possible mechanism of protection in Fe-NTA-mediated renal oxidative damage in rats. Our study proved that they both exhibit a renoprotective potential and, hence. they can be used as value-added agents for managing renal diseases as well as conditions associated with oxidative overload.