Chemometrics-Enhanced Micelle-Mediated Extraction Spectrophotometric Method for Simultaneous Determination of Cu2+ and Zn2+ in Medicinal Plant, Rice and Water Samples Using Continuous Wavelet Transform

Abstract

A new micelle-mediated extraction method for the pre-concentration of trace amounts of Cu2+ and Zn2+ as a prior step to their simultaneous spectrophotometric determination has been developed in various samples. The analytes were complexed with new synthesized ligand 4,4′-((4-chlorophenyl)methylene)bis(1,3-diphenyl-1H-pyrazol-5-ol) (CPBMPY) and Triton X-100 was added as a extraction agent. The optimal reaction and extraction conditions were optimized and the analytical characteristics of the method were obtained. The detection limit of the method was 0.90 and 0.30 ng mL−1 for Cu2+ and Zn2+, respectively. Continuous wavelet transformation (CWT) of visible spectra as a very simple and accurate method was developed for the simultaneous determination of binary mixtures of Cu2+ and Zn2+ ions. A zero-crossing technique was applied on the transformed signals and the constructed calibrations were tested by analyzing the composition of the different binary mixtures. The proposed procedure successfully was applied to analysis of water, rice, and medicinal plant and reference material samples. The amounts of metal ions obtained by the proposed methods were in good agreement with those obtained by Graphite furnace atomic absorption spectrometry.