Abstract
For structural identification of minute quantities of oligosaccharides derived from glycoconjugates, chromatography or electrophoresis may be the only practical methods available. In reality, however, to analyze and identify a compound or a mixture of compounds by any separation method (HPLC, CZE, etc.) is to compare the unknown compounds with the known compounds directly or indirectly. For example, in amino acid analysis by cation exchange chromatography or by RP-HPLC, all the amino acids expected to be present are completely separated under the standard conditions, and the peaks from any unknown samples can be assigned to one of the peaks in the standard mixture. Thus structural identification is by a tacit agreement that there is nothing else in the unknown sample but the expected components which can be completely separated.
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