Chemometric-Based Optimization of Ionic Liquid-Based Dispersive Liquid-Liquid Microextraction for Separation and Preconcentration of Erythrosine from Real Matrices

Abstract

In this research paper, a simple and economic ionic liquid-based liquid-liquid microextraction (IL-DLLME) procedure was developed to ensure efficient and rapid separation and preconcentration of erythrosine from cosmetic and food samples. Important parameters such as IL volume, temperature, acetone volume, ultrasonic time and pH that may affect the IL-DLLME procedure have been optimized by central composite design (CCD) based on response surface methodology (RSM). The optimum values of IL volume, temperature, acetone volume, ultrasonic time and pH were determined as 440 µL, 35 oC, 120 µL, 9 min and 4.2, respectively. Using these optimum conditions, some analytical data obtained for erythrosine were listed below. Working range, limit of detection and enrichment factor were 2-400 ng mL-1, 0.65 ng mL-1 and 79, respectively. The relative standard deviation (RSD%) was 2.4% for 50 ng mL-1 of erythrosine. The recovery obtained in the analysis of real samples was in the range of 93.2-108.5%. The analytical data obtained showed that the IL-DLLME procedure was successfully applied to the selected samples