Abstract
Introduction: Paederia foetida and Vitis vinifera were reported to have antioxidant activities due to their polyphenolic phytochemical contents. Arbutin may be included as active compounds in the plants. The chemometric analysis is used to identify the similarity of functional groups found in arbutin and that are in Paederia foetida and Vitis vinifera. Materials and Method: The ethanol extracts of Paderia foetida and Vitis vinifera were subjected to compound partition and characterization. Then a liquid-liquid partition is carried out using n-hexan and water solvents, followed by ethyl acetate and water partitions. This procedure is carried out continuously until the top layer is cleared. Partitioned compounds were analyzed using FTIR spectroscopy to identify functional groups. Furthermore, it was analyzed using the chemometric method (Principal Component Analysis and Cluster Analysis). Results: From the results of FTIR spectroscopy, functional groups in Paederia foetida and Vitis vinifera were identical to functional groups found in arbutin. PCA Analysis was achieved using principal component accounting an eigenvelue about 99,3 % of the total variance. Conclusions: CA analysis showed that compounds in Paederia foetida (Euclidean distance 0.537) and Vitis Vinifera (Euclidean distance 1.157) may be similar with arbutin.
Highlights
Paederia foetida and Vitis vinifera were reported to have antioxidant activities due to their polyphenolic phytochemical contents
The chemometric analysis is used to identify the similarity of functional groups found in arbutin and that are in Paederia foetida and Vitis vinifera
From the results of Fourier Transform Infrared (FTIR) spectroscopy, functional groups in Paederia foetida and Vitis vinifera were identical to functional groups found in arbutin
Summary
Arbutin (β-arbutin) is a natural whitening compound of glycosylated derivatives from hydroquinone as tyrosinase inhibitors. This compound can be obtained from Ericaceae (berberry, strawberry, huckleberry), Saxifragaceae, Asteraceae, Rosaceae, Lamiaceae, Apiaceae and is found in pears (Pyrus communis L.). In addition wine has antioxidant, anticancer, anti-inflammatory, antiaging and antimicrobial effects. Another content contained in grape leaves is arbutin, mentioned that arbutin accumulates in grape leaves.[7,8] It is necessary, to identify and characterize arbutin compounds contained in Paederia foetida and Vitis vinifera using FTIR spectrophotometers and chemometric analysis
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