Chemometric Analyses for the Characterization of Raw and Stir-Frying Processed Drynariae Rhizoma Based on HPLC Fingerprints.

Jing Hu,Beibei Qin,Jiali Wang,Xia Li,Lizhi Wang,Marinella De leo

doi:10.1155/2021/6651657

Abstract

The processing of traditional Chinese medicine (TCM) is a necessary practice and usually occurs before most herbs are prescribed. According to Chinese medicine theory, raw (RDR) and stir-frying processed (PDR) Drynariae Rhizoma have different clinical applications. The purpose of this study was to establish HPLC fingerprints coupled with chemometric methods to evaluate the differences between RDR and PDR. Multivariate chemometric methods were applied to analyze the obtained HPLC fingerprints, including hierarchical cluster analysis (HCA), principle components analysis (PCA), and partial least squares discriminant analysis (PLS-DA). The results indicated that RDR and PDR samples showed a clear classification of the two groups, and seven chemical markers having great contributions to the differentiation were screened out. The findings suggested that 5-hydroxymethyl-2-furaldehyde (5-HMF) with a variable importance in the project (VIP > 1) can be used to differentiate between RDR and PDR. Moreover, 5-HMF, naringin, and neoeriocitrin were determined to evaluate their contents in RDR and PDR. The chemometrics combined with the quantitative analysis based on HPLC fingerprint results indicated that stir-frying processing may change the contents and types of components in Drynariae Rhizoma. These changes are probably responsible for the various pharmacological effects of RDR and PDR.

Highlights

Gu-Sui-Bu (Drynariae Rhizoma, DR) is derived from the dried rhizome of Drynaria fortunei (Kunze) J
Crude DR products are densely covered with scales, not easy to remove, and their textures are hard and tough, which are not easy to crush and extract effective ingredients. ere are two common forms of DR in the medicinal market, including raw DR (RDR) and stirfrying processed DR (PDR)
Methodological Evaluation. e method was validated for precision, repeatability, and stability. e relative retention time (RRT) and relative peak area (RPA) were calculated by using naringin as the reference peak. e precision was evaluated by injecting the same sample solution for six times. e relative standard deviation (RSD) for RRT and RPA was less than 1.3% and 1.9%, which indicated good precision of the method. e repeatability was determined by analyzing six independently extracted samples from the same batch of DR

Summary

Introduction

Gu-Sui-Bu (Drynariae Rhizoma, DR) is derived from the dried rhizome of Drynaria fortunei (Kunze) J. According to TCM theory, processing of Chinese Materia Medica is an essential technique, which can improve therapeutic efficacy and reduce toxicity, drastic properties, or side effects. Crude DR products are densely covered with scales, not easy to remove, and their textures are hard and tough, which are not easy to crush and extract effective ingredients. Ere are two common forms of DR in the medicinal market, including raw DR (RDR) and stirfrying processed DR (PDR). RDR is obtained from dried rhizome of D. fortunei, and impurities and ashes are removed. PDR is obtained by stir-frying DR with sand [4, 5]. After stir-frying processing, the drugs are expanded by heat, the textures are crisp, and the villi are scorched and easy to remove, so the drugs are beneficial to decoct effective ingredients

Methods

Results

Conclusion