Chemokinetic and chemotactic activity of various prostaglandins for neutrophil granulocytes

Abstract

Prostaglandins (PGs) of the E, A, and F type were assayed for chemokinetic and chemotactic activity in vitro. In the absence of bovine serum albumin (BSA) which is chemokinetically active, PGs of the F type (10 −7 to 10 −5 M) induced a chemokinetic and chemotactic response in rabbit peritoneal neutrophils. Under the same conditions, PGs of the E and A type had no significant effect on cell locomotion. However, in the presence of BSA (1 mg/ml), PGE and PGA induced a chemotactic response, thus indicating that they lack chemokinetic but possess chemotactic activity. In the absence of BSA, PGE 1 caused an increase in cyclic AMP, which was not observed when BSA was present. This supports previous findings that an increase of cyclic AMP is associated with inhibition of chemotactic locomotion. Since PGs are released by phagocytosing leukocytes and since albumin is generally available in inflamed tissues, the results suggest that PGs may contribute to the inflammatory accumulation of neutrophils.