Chemokines and pituitary functions

Abstract

Event Abstract Back to Event Chemokines and pituitary functions Tullio Florio1* 1 University of Genova Viale Benedetto XV, Department of Oncology Biology and Genetics, Italy Stromal cell-Derived Factor 1 (SDF1) and its receptor, CXCR4, were reported to play a regulatory roles in extra-lymphoid cells and in particular in the Central Nervous System. SDF1 and CXCR4 mRNA expression was identified in many tumor histotypes and were involved in proliferation, migration, metastasization and neoangiogenesis. To date no data are available on the role of SDF1/CXCR4 in normal pituitary function and in the genesis or deveoplment of pituitary adenomas. We previously reported SDF1 to induce GH and prolactin secretion and proliferation in GH4C1 rat pituitary adenoma cells, through the activation of multiple intracellular systems. In this study, we analyzed the expression and the biological significance of the SDF1/CXCR4 ligand/receptor pair in human pituitary adenomas and in human normal pituitaries.The expression of SDF1 and CXCR4 in 56 human pituitary adenomas (25 GH-secreting, GHoma, and 31 clinically non-functioning, NFPA) and 4 human normal pituitaries was determined by RT-PCR, immunohistochemistry and confocal immunofluorescence. The proliferative effect of SDF1 was evaluated in 8 fibroblast-free human pituitary adenoma cell cultures.CXCR4 mRNA was expressed in 92% of GHomas and 81% of NFPAs, while SDF1 was identified in 63% and 78% of GHomas and NFPAs, respectively, being the co-expression of both molecules the most common event. Immunostaining for CXCR4 and SDF1 demonstrated a strong homogenous labeling in all tumoral cells in both GHomas and NFPAs. In normal tissues, CXCR4 and SDF1 were express only in a subset of anterior pituitary cells, with a lower expression of SDF1 compared to its cognate receptor. CXCR4 and SDF1 were not confined to a specific cell population in the anterior pituitary, but co-localized with discrete subpopulations of GH, prolactin and ACTH-secreting cells. Conversely, most of the SDF1-containing cells expressed CXCR4. No colocalization of CXCR4 and SDF1 was observed within the tumor mass with CD34 (endothelial cells) or in normal pituitary with S100 (folliculostellate cells) or CD34, indicating a selective expression with pituiticites. In 6 out of 8 pituitary adenoma primary cultures, SDF1 induced a statistically significant increase in DNA synthesis that was prevented by the treatment with the CXCR4 antagonist AMD3100 or somatostatin.CXCR4 and SDF1 are over-expressed in human pituitary adenomas and CXCR4 activation may contribute to pituitary cell proliferation and, possibly, to adenoma development in humans. Conference: 3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009. Presentation Type: Oral Presentation Topic: Symposium 17 – Chemokines: new modulators in brain and pituitary functions Citation: Florio T (2009). Chemokines and pituitary functions. Front. Neurosci. Conference Abstract: 3rd Mediterranean Conference of Neuroscience . doi: 10.3389/conf.neuro.01.2009.16.071 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Nov 2009; Published Online: 20 Nov 2009. * Correspondence: Tullio Florio, University of Genova Viale Benedetto XV, Department of Oncology Biology and Genetics, Genova, Italy, tullio.florio@unige.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tullio Florio Google Tullio Florio Google Scholar Tullio Florio PubMed Tullio Florio Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.