Abstract
背景与目的趋化因子激素受体（CC chemokine receptor 7, CCR7）与非小细胞肺癌（non-small cell lung cancer, NSCLC）的淋巴结转移密切相关，但CCR7促进其淋巴结转移的机制尚不明了。本研究通过观察CCR7和MMP-9在NSCLC组织中的表达和相互关系，探讨CCR7促进NSCLC淋巴结转移的机制。方法应用免疫组织化学染色（SP法）检测90例NSCLC组织中CCR7、MMP-9的表达；将BE1细胞经趋化因子CCL19处理24 h后，应用RTPCR和Western blot方法检测MMP-9 mRNA和蛋白表达水平。结果免疫组织化学结果显示：CCR7主要表达于癌细胞胞质和（或）胞膜，MMP-9主要表达于癌细胞胞质，NSCLC中CCR7、MMP-9阳性表达率分别为70%（63/90）和65.5%（59/90），χ2检验显示CCR7和MMP-9表达与NSCLC的临床病理分期（P=0.003, P=0.001）和淋巴结转移（P=0.004, P=0.003）密切相关，而与年龄、组织学类型、分化程度无关（P > 0.05）。此外，CCR7和MMP-9表达正相关（r=0.342, P=0.001）。CCL21处理组BE1细胞后MMP-9 mRNA和蛋白水平均上调（P < 0.05）。结论CCR7和MMP-9表达与NSCLC侵袭转移密切相关，CCL19/CCR7通过上调NSCLC中MMP-9表达促进其转移。
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