Abstract
AbstractThe aim of this study was to produce stearidonic acid (SDA, 18:4n‐3) omega‐3 concentrates from 25 % SDA soybean oil (SDASO) by enzymatic acidolysis. Substrates were prepared by chemical and enzymatic hydrolysis of SDASO. A 62 % SDA free fatty acid mixture (SDA‐FFA) was obtained by low temperature crystallization of the chemical hydrolyzate while partial hydrolysis of SDASO by non‐immobilized lipase AY 30 (Candida rugosa) yielded a 51 % SDA acylglycerol mixture (SDA‐GLY). Reaction conditions for acidolysis between SDA‐FFA and SDA‐GLY were optimized using response surface methodology (RSM). Incorporation of SDA into SDA‐GLY by immobilized Lipozyme RM IM (Rhizomucor miehei) and non‐immobilized Lipomod 34P‐LO34P (C. cylindracea [rugosa]) lipases were modeled under varying levels of the substrate molar ratio (Sr), temperature (T), and time (t). Optimal conditions for production of a 60 % SDA concentrate were predicted to be Sr = 4.8, T = 65 °C and t = 8 h for Lipozyme RM IM and Sr = 5.0, T = 43 °C and t = 48 h for Lipomod 34P‐L034P. The model was verified experimentally by gram scale synthesis under optimal conditions which resulted in the production of 59.98 and 58.98 % SDA concentrates (≥96 % triacylglycerols) by Lipozyme RM IM and Lipomod 34P‐L034P, respectively.
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