Abstract
In this work, the feasibility of chemoenzymatically transforming biomass-derived D-xylose to furfuryl alcohol was demonstrated in a tandem reaction with SO42−/SnO2-CS chemocatalyst and reductase biocatalyst in the deep eutectic solvent (DES)–water media. The high furfural yield (44.6%) was obtained by catalyzing biomass-derived D-xylose (75.0 g/L) in 20 min at 185 °C with SO42−/SnO2-CS (1.2 wt%) in DES ChCl:EG–water (5:95, v/v). Subsequently, recombinant E.coli CF cells harboring reductases transformed D-xylose-derived furfural (200.0 mM) to furfuryl alcohol in the yield of 35.7% (based on D-xylose) at 35 °C and pH 7.5 using HCOONa as cosubstrate in ChCl:EG–water. This chemoenzymatic cascade catalysis strategy could be employed for the sustainable production of value-added furan-based chemical from renewable bioresource.
Highlights
With the rapid consumption of fossil-fuels, together with the growing global warming and environmental concerns, lignocellulosic biomass is regarded as ideal and promising alternative for producing biofuels and bio-based chemicals [1,2]
Choline chcloride (ChCl), ethylene glycol (EG), D-xylose, glucose, SnCl4 ·5H2 O, sodium formate (HCOONa), NADPH, sulfuric acid (H2 SO4 ), and other chemicals were purchased from Sino pharm Group Chemical Reagent Co., Ltd. (Shanghai, China)
The acid-treated Corn stalks (CS) (AT-CS) was mixed with SnCl4 -5H2 O and anhydrous ethanol, ammonia was slowly dripped into the mixture; the resulting colloidal solution was dried in an oven, the dried solid powder was dried after sulfonation in dilute H2 SO4 and calcined in a muffle furnace to obtain SO4 2− /SnO2 -CS [24]
Summary
With the rapid consumption of fossil-fuels, together with the growing global warming and environmental concerns, lignocellulosic biomass is regarded as ideal and promising alternative for producing biofuels and bio-based chemicals [1,2]. Biomass or biomassderived D-xylose has been industrially used for the production of furfural (FAL) with acid catalyst via dehydration reaction [1,3]. The production of FOL was conducted via chemical-enzymatic cascade conversion of biomass-derived D-xylose in a tandem reaction by sequential catalysis with biomass-based solid acid SO4 2− /SnO2 -CS chemocatalyst and E. coli CF whole-cells biocatalyst in DES ChCl:EG–water system. The effects of various chemical reaction parameters (e.g., ChCl:EG dosage, SO4 2− /SnO2 -CS dose, performance temperature, and catalytic time) on the production of FAL were investigated using biomass-derived D-xylose as feedstock. One-pot chemical-enzymatic synthesis of FOL from biomass-derived D-xylose was demonstrated by sequential dehydration with bio-compatible solid acid SO4 2− /SnO2 -CS and bio-reduction with recombinant E. coli
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