Abstract
Abstract A novel class of complex oligosaccharides with a backbone made of twelve β-(1→4)- d -glucosyl units that may be regularly substituted on their primary position with α- d -xylosyl or β- d -(1→2)-galactosyl-α- d -xylosyl residues were readily synthesized using enzymatic hydrolysis of the plant polysaccharide xyloglucan (XG), followed by chemical modifications of the well-defined oligosaccharides and their enzymatic coupling catalyzed by the Cel7B E197A glycosynthase from Humicola insolens. These complex oligosaccharides proved useful for a better understanding of the effects of structural variation of xyloglucan on interactions with bacterial microcrystalline cellulose (BMCC).
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