Chemo-enzymatic synthesis of the human angiogenin gene. Construction of bacterial strains, producers of human angiogenin. Elaboration of a technology for the purification and preparation of angiogenin

Abstract

Chemo-enzymatic synthesis of the gene of human angiogenin, a stimulator of blood vessel growth, was performed for the first time. Cloning of the human angiogenin gene in several expressing vector systems was carried out, and several bacterial strains, producers of human angiogenin were constructed. In particular, strains, producers of “angiogenin-β-galactosidase” and “angiogenin-domains of staphylococcal protein A hybrid proteins” were obtained, as well as a bacterial strain efficiently expressing free angiogenin. Large-scale preparation of purified recombinant human angiogenin was accomplished using theE.coli JM103 pRITA16 producer strain constructed, and the biological activity of the resulting preparation was studied.