Abstract
Partially and fully N-deacetylated 4-methylumberlliferyl chitobioside (1 ) derivatives, such as GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3 ), and (GlcN)2-UMB (4), were synthesized using chemo- and enzymatic procedure. Fluorescent aglycon was released from the chitobiosides 1, 2 and 3 by the action of chitinase. These UMB glycosides of heterochitobiose were versatile probes for the investigation of substrate binding chitinase from various sources.
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