Chemo-accumulation without changes in membrane potential in the microstome form of the ciliate Tetrahymena vorax

Abstract

The swimming behaviour of ciliates is mainly determined by membrane potential and transmembrane fluxes. In a chemical gradient, swimming ciliates may approach or move away from the source. Based on experiments on Paramecium, it is generally assumed that chemical attractants and repellents affect the swimming behaviour of ciliates by specific changes in the membrane potential. We have examined whether there is a causal relationship between membrane potential and chemo-accumulation in the microstome form of the polymorphic ciliate Tetrahymena vorax. Effects of chemo-attractants on the membrane potential of Tetrahymena have not been previously reported. Microstome T. vorax cells aggregated close to a point source of l-cysteine and the complex meat hydrolysate proteose peptone. Chemo-accumulated cells displayed a significantly higher turning frequency than control cells at a similar cell density. A concentration of 20 mmol l(-1) l-cysteine did not evoke any detectable change in the membrane potential whereas 1% proteose peptone depolarised the cells by ∼12 mV. This is contrary to the current model, which predicts agents that induce a moderate depolarisation to be repellents. A solution of 1% proteose peptone contains 21 mmol(-1) Na(+). A solution of 21 mmol(-1) NaCl without organic compounds also caused ∼12 mV depolarisation but had no aggregating effect on the cells. Collectively, the electrophysiological and behavioural data indicate that chemo-accumulation in the microstome form of T. vorax is not governed obligatorily by the membrane potential. We thus suggest that the simple membrane potential model for chemokinesis in Paramecium may not be valid for T. vorax.