Abstract
Acrylamide monomer has been shown to be a potent neurotoxin in animals and man. Methods reported for the analysis of acrylamide are based on the derivatization to 2,3-dibromopropionamide and analysis by gas chromatography (GC) with a column packed with a free fatty acid phase (FFAP) (Carbowax 20 M terminated with 2-nitroterephthalic acid) and an electron-capture detector. Using gas chromatography—mass spectrometry (GC—MS), we found that 2,3-dibromopropionamide is not a stable derivative for GC analysis. At low concentrations, it is converted to 2-bromopropenamide at the front end of the packed column. At high concentrations, both the mono- and di-bromo derivatives can be detected on a DB-5 fused-silica capillary column. On an inert FFAP capillary column, the derivative does not decompose to 2-bromopropenamide nor is it eluted in a symmetrically shaped peak. Conversion to the more stable 2-bromopropenamide can be achieved by adding triethylamine to 2,3-dibromopropionamide prior to GC analysis. This conversion is quantitative and reproducible. This derivative can be analyzed on a capillary column with improved resolution and detectability of at least 330 ppt (0.6 pg injected on column).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
